Plasmid

Part:BBa_K5048042

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


plasmid ccdBA

We used pET-28a as a backbone.The expression of ccdB and ccdA is separately regulated by T7 promoter and araBAD promoter in order to achieve flexible control.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5817
    Illegal BamHI site found at 582
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 990
    Illegal NgoMIV site found at 4037
    Illegal NgoMIV site found at 4197
    Illegal NgoMIV site found at 5785
    Illegal AgeI site found at 417
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 245
    Illegal SapI site found at 399
    Illegal SapI.rc site found at 3116


[edit]
Categories
Parameters
None